Journal: Scientific Reports

Article Title: Mitochondrial sub-cellular localization of cAMP-specific phosphodiesterase 8A in ovarian follicular cells

doi: 10.1038/s41598-019-48886-8

Figure Lengend Snippet: Immunolocalization in mitochondrial isolated fractions from granulosa cells by immunofluorescence using anti-P450scc (green, B , United States Biological), anti-PDE8A (green, E , Proteintech) and MitoTracker (orange, A , D ). Merge signals are shown in ( C , F ). The signal was calibrated with non-specific IgG. Magnification is shown as a bar of 10 µm.

Article Snippet: The samples were washed twice in PBS solution, centrifuged at 14,000 × g for 5 min to eliminate residual markers, fixed with 4% (w/v) PFA in 0.2% Triton X-100 for 10 min in the dark at room temperature, blocked with 5% BSA in PBS for 1 h, then kept at 4 °C overnight with PDE8A polyclonal antibody (Proteintech product number 13956-1-AP, 1:500 in PBS) or porcine P450scc polyclonal antibody (United States Biological, Salem, MA, product number 362774, 1:100 in PBS).

Techniques: Isolation, Immunofluorescence

Journal: Journal of Bone and Mineral Research

Article Title: Mapping of Human Autoantibody Binding Sites on the Calcium-Sensing Receptor

doi: 10.1359/jbmr.090703

Figure Lengend Snippet: Synthetic peptide ELISAs with patient and control sera. Patient and control sera were analyzed for antibody binding to synthetic peptides in an ELISA format, as detailed in Materials and Methods. The Ab indices are shown for APS1 ( n = 14) and AHH ( n = 3) patient sera and for control ( C ) ( n = 10) serum samples analyzed in ELISA experiments against synthetic CaSR peptides 41–69, 114–126, 214–238, 344–358, and 374–391.

Article Snippet: Anti-CaSR rabbit polyclonal antibody against a synthetic peptide corresponding to amino acids 12–27 of the rat CaSR was purchased from Alexis Biochemicals (Nottingham, UK).

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay