Journal: Brain, behavior, and immunity

Article Title: Bone marrow mononuclear cells exert long-term neuroprotection in a rat model of ischemic stroke by promoting arteriogenesis and angiogenesis

doi: 10.1016/j.bbi.2013.07.010

Figure Lengend Snippet: Transplanted BMMNCs incorporate into brain vessels and promote angiogenesis. Representative images of leptomeningeal anastomoses on days 14, 28 and 42 (A–I) and intracranial parenchymal microvessels on day 42 (J–L) stained with anti-BrdU antibody (green) and anti-CD31 antibody (red) (scale bar = 50 μm). (M–N) Quantification showed an increase in the number of BrdU-positive endothelial cells (ECs, M) and smooth muscle cells (SMCs, N). *p<0.05 vs. the number of BrdU-positive ECs or SMCs on day 14 or 42; n=6/time point.

Article Snippet: Mouse anti-BrdU monoclonal antibody (BBI, Shanghai, China), rabbit anti-rat polyclonal antibody against smooth muscle actin (α-SMA; BBI), and rabbit anti-rat polyclonal antibody against CD31 (BBI) were used as primary antibodies at a dilution of 1:250.

Techniques: Staining

Journal: Journal of Bone and Mineral Research

Article Title: Mapping of Human Autoantibody Binding Sites on the Calcium-Sensing Receptor

doi: 10.1359/jbmr.090703

Figure Lengend Snippet: Synthetic peptide ELISAs with patient and control sera. Patient and control sera were analyzed for antibody binding to synthetic peptides in an ELISA format, as detailed in Materials and Methods. The Ab indices are shown for APS1 ( n = 14) and AHH ( n = 3) patient sera and for control ( C ) ( n = 10) serum samples analyzed in ELISA experiments against synthetic CaSR peptides 41–69, 114–126, 214–238, 344–358, and 374–391.

Article Snippet: Anti-CaSR rabbit polyclonal antibody against a synthetic peptide corresponding to amino acids 12–27 of the rat CaSR was purchased from Alexis Biochemicals (Nottingham, UK).

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay